Characterization of a novel Pseudomonas aeruginosa phage species:PaYy-2

Abstract

A novel Pseudomonas aeruginosa phage, designated as PaYy-2, has been isolated from a lake in China and its genome meticulously sequenced and characterized. The PaYy-2 phage possesses a 92,348 base pair double-stranded DNA genome that encodes for 168 proteins, exhibiting a 68% similarity to the genome of the known Pseudomonas phage YS35. Phylogenetic analysis reveals that the putative RNA polymerase of PaYy-2 shares a striking 94% similarity with that of Pseudomonas phage SRT6, while the putative terminase large subunit bears a 96% resemblance to the phage YS35, both of which are classified under the Pakpunavirus genus. Morphological examination via transmission electron microscopy (TEM) has provided insights into the phage's tail and particle morphology. These collective findings underscore the classification of PaYy-2 as a distinct species within the Pakpunavirus genus of the family Myoviridae.