Differentially Expressed Genes and Cerna Network of Early Subperiosteal Osteogenesis in Rabbit Skull Defect Models

Authors

  • Wenxue Wang Department of Oral Implantology, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong Province, China
  • Baodong Zhao Department of Oral Implantology, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong Province, China
  • Hao Xu Department of Oral Implantology, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong Province, China
  • Li Zhen Department of Oral Implantology, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong Province, China
  • Xin Li* Department of Oral Implantology, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong Province, China

Keywords:

early subperiosteal osteogenesis, differentially expressed genes, competing endogenous RNA, functional enrichment

Abstract

Objective: To explore potential critical genes and identify ceRNA network participating in early stage of sub periosteal osteogenesis.

Materials and Methods: Rabbit skull defect models were constructed. The periosteum of defects and control site were obtained 3 weeks post surgery, and a bioinformatics method was used to analyze differentially expressed (DE) genes and construct a ceRNA regulatory network.

Results: In total, 332 lncRNAs, 22 circRNAs, 37 miRNAs and 1141 mRNAs were differentially expressed. The functional enrichment suggested the immune response, cell-cell interaction, cell proliferation and differentiation, and angiogenesis were associated with wound healing and osteogenesis at an earlier stage. CeRNA networks with 39 lncRNA/ 33 miRNAs/ 51mRNAs and 8 circRNAs/ 9 miRNAs/ 46 mRNAs. were constructed respectively.

Conclusion: Expression gene profiles were significantly altered in periosteum during early healing stages of bone regeneration. This study revealed potentially important genes, pathways and ceRNA regulatory networks participating in intramembranous osteogenesis.

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Published

2024-12-30

Issue

Vol. 94 No. 6 (2024): Volume 94, Issue 6

Section

Original Articles

How to Cite

Wang, Wenxue, Baodong Zhao, Hao Xu, Li Zhen, and Xin Li* , trans. 2024. “Differentially Expressed Genes and Cerna Network of Early Subperiosteal Osteogenesis in Rabbit Skull Defect Models”. Human Biology 94 (6): 891-98. https://www.humbiol.org/Home/article/view/181.

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