ALA/LA Inhibited Renal Tubulointerstitial Fibrosis of DKD db/db Mice Induced by Oxidative Stress

Authors

  • Mingxia Jiang* Department of Clinic Nutrition, The Fourth Affiliated Hospital of Soochow University, Suzhou 215123, China
  • Hong Sun Department of Endocrinology, The Fourth Affiliated Hospital of Soochow University, Suzhou 215123, China
  • Haifen Zhang School of Tourism and Culinary Science, Yangzhou University, Yangzhou 225127, China
  • Yin Cheng Henan Vocational College of Nursing, Anyang 455000, China
  • Chengkai Zhai* School of Public Health, Southeast University, Nanjing, 210009, China

Keywords:

α-linolenic acid, linoleic acid, diabetic kidney disease, oxidative stress, tubule intersti-tial fibrosis

Abstract

Background: Diabetic kidney disease (DKD) is a leading cause of end-stage renal disease. Its progression is caused by various pathological mechanisms, including oxidative stress (OS), inflammation, and fibrosis. This study aimed to explore the effects of alpha-linolenic acid (ALA)/ linoleic acid (LA) on preventing and delaying the progression of interstitial fibrosis and improving OS in DKD mice.

Methods: Male eight-week-old db/db mice were randomly allocated to either the DKD model group, the low-dose ALA/LA group (250 mg/kg·d), the high-dose ALA/LA group (500 mg/kg·d), or the control group, consisting of db/m mice. After 12 weeks of ALA/LA intervention, blood urea nitrogen, blood glucose, and urine protein levels were signifi-cantly lower in db/db mice than in the control group.

Results: ALA/LA enhanced SOD and CAT levels and reduced reactive oxygen species and MDA production. Furthermore, db/db mice in the intervention group had lower mRNA and protein expression levels of p38, p-p38, ERK, p-ERK, /transforming growth factor-β1 (TGF-β1), and type IV collagen (ColIV) than did the model group (P<0.05).

Conclusion: ALA/LA improved recovery from injury in db/db mice by reducing OS and alleviating kidney fibrosis, especially in the tubules. The potential mechanism was that ALA/LA inhibited renal tubulointerstitial fibrosis and OS via the P-P38, P-ERK/ TGF-β1/ColIV signaling pathway

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Published

2024-11-01

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Section

Original Articles

How to Cite

Mingxia Jiang*, Hong Sun, Haifen Zhang, Yin Cheng, and Chengkai Zhai* , trans. 2024. “ALA/LA/Inhibited/Renal/Tubulointerstitial/Fibrosis/of/DKD/Db/Db/Mice/Induced/by/Oxidative/Stress”. Human Biology 94 (5): 802-9. https://www.humbiol.org/Home/article/view/161.